The purpose of this study is to examine the extent to which some factors such as trust, cultural differences, time differences, language have a significant effect on virtual team's work collaboration. Study population comprised of Jordanian Royal Medical Services; two most largest medical services were selected as a study sample; King Hussein Medical Centre and Queen Alia Medical Centre both located at Amman (the capital of Jordan). A purposive sampling technique was used to select the respondents surveyed for this study and a questionaire was used to collect primary data from the respondents, a total of 120 questionnaires were administered to potential respondents chosen from the two medical services; a total of 105 usable questionnaires were returned, giving a response rate of 87.5 %. Simple regression analysis was performed to test the study hypothesis. The study findings of this research show that there is a statistical significant effect at cultural differences, time differences and language on virtual team's collaboration, whereas, trust factor did not show a statistical significant effect. This study is delimited to only Jordanian royal medical services, as a case study, thus the results of this study cannot be generalised to other medical services.